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My résumé
Vladislav
Dolnik 409 Kent Drive Mountain
View, CA 94043-5280 Voice (H): 650-964-1952 Voice
(W): 408-737-4810 Fax
(W): 408-737-4808 E-mail
(W): vlad.dolnik@am.apbiotech.com E-mail (H): dolnik@telocity.com Homepage: http://dolnik.net
Education: Postdoctoral
1988-1989/1991-1992; Indiana
University, Department of Chemistry, Bloomington, Indiana. Ph.D.
1988; Analytical Chemistry; Institute of
Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech
Republic. (Candidatus Scientium.) M.S.
1976; Biochemistry; Masaryk University,
Brno, Czech Republic, Faculty of Science. (Rerum naturalium doctor.) B.S. 1975; Biochemistry; Masaryk University, Brno, Czech Republic, Faculty of Science. Positions: 1995
- present: Staff Scientist, Molecular Dynamics,
Sunnyvale, California. 1979 - 1995: Research Scientist/Senior Staff Scientist, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. July
1977- June 1979: Supervisor, Laboratory of Clinical Chemistry, District
Hospital, Rychnov nad Kněžnou, Czech Republic. September
1975- June 1976; Assistant Professor, Masaryk
University, Medical School, Department of Hygiene and Epidemiology, Brno,
Czech Republic.
The
most
important projects: Preparation
of gel-filled capillaries for capillary zone electrophoresis; Preparation
of hydrolytically stable wall coating for capillary electrophoresis; New
technologies for preparation of gel-filled capillaries and coated capillaries
for capillary zone electrophoresis and isoelectric focusing; Separation
of biopolymers by electromigration methods; Microfabrication,
separations on microchip, connection to MS; Electromigration
separation methods in clinical chemistry and medicinal chemistry; Development
of an advanced instrumentation for capillary electrophoresis and
isotachophoresis; Trace
and ultratrace analysis by electromigration methods; Clinical
chemistry; metabolism of organic acids; evaluation of metabolic acidosis. Human
interferon and its physicochemical properties.
Grants: NIH
SBIR grant: 1R
43 H601563-02 Sieving media for DNA sequencing with long read lengths (600
k$ direct cost, 800 k$ total cost) Patents: M. Novotny, K.A. Cobb, and V. Dolník: Suppression of
electroosmosis with hydrolytically stable coatings. U. S. Patent # 5,074,982, December 24, 1991. M. Novotny, V. Dolník, and K.A. Cobb: Capillary gels
formed by spatially progressive polymerization using migrating initiator. U.S.
Patent # 5,080,771, January 14, 1992. M. Novotny, K.A. Cobb, and V. Dolník:
Suppression of electroosmosis with hydrolytically stable coatings. U. S. Patent
# 5,143,753
, September 1, 1992. Australian Patent # 639873,
October 17, 1991. U.K. Patent # 2251245, October 8, 1994. V. Dolník, M. Chiari: Compounds for molecular
separations. U.S. Patent # 6,074,542, June 13, 2000.
The
most
frequently cited publications: 237× K.A. Cobb, V. Dolník and M. Novotny:
Electrophoretic separation of proteins in capillaries with hydrolytically stable
surface structures. Anal.
Chem., 62 (1990) 2478- 2483. 127×
P. Boček, M. Deml, P. Gebauer and V. Dolník: Analytical
Isotachophoresis. VCH Verlagsgesellschaft, Weinheim- New York, 1988. 76×
V. Dolník, K.A. Cobb and M. Novotny: Capillary zone electrophoresis of
dilute samples with isotachophoretic preconcentration. J. Microcol. Sep., 2
(1990) 127- 131. 67×
V. Dolník, J. Liu, J.F. Banks, Jr., M. Novotny and P. Boček:
Capillary zone electrophoresis of oligonucleotides. Factors affecting
separation. J. Chromatogr., 480 (1989)
321- 330. 51×
J. Liu, V. Dolník, Y.-Z. Hsieh and M. Novotny: Experimental evaluation
of the separation efficiency in capillary electrophoresis using open tubular and
gel-filled columns. Anal. Chem., 64
(1992) 1328- 1336. 44×
V. Dolník, K.A. Cobb and M. Novotny: Preparation of polyacrylamide
gel-filled capillaries for capillary electrophoresis. J.
Microcol. Sep., 3 (1991) 155- 159. 41×
P. Boček, P. Gebauer, V. Dolník and F. Foret: Recent developments
in isotachophoresis. J. Chromatogr., 334
(1985) 157- 195. 35×
V. Dolník and M. Novotny: Capillary electrophoresis of DNA fragments in
entangled polymer solutions: A study of separation variables. J. Microcol. Sep., 4 (1992) 515- 519. 34×
V. Dolník and M. Novotny: Separation of amino-acid homopolymers by
capillary gel electrophoresis. Anal.
Chem., 65 (1993) 563- 567. 31×
V. Dolník, M. Deml and P. Boček: Large sample volume preseparation
for trace analysis in isotachophoresis. J.
Chromatogr., 320 (1985) 89- 97. 29× V.
Dolník: Capillary zone electrophoresis of serum proteins. Study of separation
variables. J. Chromatogr., 709 (1995)
99- 110. Outside
Services: Member of organizing committee of HPCE’99. Associate director of
California Separation Science Society (2000). Guest Editor, special
issue of Electrophoresis, CE wall coatings and sieving matrices (No. 4, 2001) Member of editorial
board: Journal
of MicroColumn Separations (since 1994)
Electrophoresis
(since 1999) Electronic
list owner: CZE-ITP,
electronic conference on electrophoretic capillary methods (since 1993) Referee: Professional
Memberships: Czech Medical Society (1977) Czech
Chemical Society (1988) Czechoslovak
Society of Arts and Sciences (1990) The
New York Academy of Sciences (1995) American
Chemical Society (1995) American
Association for the Advancement of Science (1995) California
Separation Science Society (1997) American
Society for Mass Spectrometry
(2000)
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